Anti-CDKN2A Monoclonal Antibody
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Synonyms:
ARF, CDK4 inhibitor P16 INK4, CDK4I, CDKN2, CDKN2A, Cell cycle negative regulator beta, CMM2, Cyclin dependent kinase 4 inhibitor A, Cyclin dependent
Entrez Gene ID:
(Murine) 12578
UniProt ID:
P51480
| Details | |
| Host / Isotype: | Mouse / IgG2b |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | 5-C3-1 |
| Species Reactivity: | Murine (Ms) |
| Immunogen: | Synthetic peptide containing amino acids 54-75 of the murine p19ARF. |
| Ordering Information | ||||
| Pierce Anti-CDKN2A Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| MA1-16665 | 100 µl | $342.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µl of protein A purified antibody (1 mg/ml) in Tris-glycine, 150mM NaCl with 0.05% sodium azide. |
| Applications | Dilution |
| Immunofluorescence (IF) | Assay Dependent |
| Immunohistochemistry (IHC) | Assay Dependent |
| Immunoprecipitation (IP) | Assay Dependent |
| Western Blot (WB) | 1 ug/ml |
| Product Specific Information |
| MA1-16665 detects p19ARF in mouse samples. It does not react with human or Golden hamster. Armenian hamster has not been tested. MA1-16665 has successfully been used in Western blot, immunoprecipitation, immunofluorescence, and immunohistochemistry applications. The p19ARF molecular weight is 19 kDa. The MA1-16665 immunogen is amino acids 54-75 of murine p19ARF. It is recommended to use a rat secondary in the outlined applications. Figure 1 illustrates immunohistochemical staining of sections from a wild type mouse testis probed with MA1-16665 (green). Sections were stained with DAPI to illustrate the position of cell nuclei (blue). Figure 2 illustrates Western blot detection of endogenous p19Arf using MA1-16665. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (lane 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (lane 2), were resolved by SDS-PAGE. |
| General Information |
| In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression. |
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Immunohistochemistry with anti-CDKN2A Monoclonal Antibody [5-C3-1] (MA1-16665)
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Western Blot with anti-CDKN2A Monoclonal Antibody [5-C3-1] (MA1-16665)
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(This product is for In Vitro experimental use only.)
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PO
Box 117, Rockford, IL 61105 USA |
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