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HIF-1 ALPHA ANTIBODIES > HIF-1 ALPHA MONOCLONAL ANTIBODY MA1-16504
Anti-HIF-1 alpha Monoclonal Antibody
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Synonyms:
Hypoxia Inducible Factor 1 alpha, ARNT interacting protein, ARNT2, MOP1, PASD8
Entrez Gene ID:
(Human) 3091
UniProt ID:
Q16665
| Details | |
| Host / Isotype: | Mouse / IgG2b |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | H1alpha67 |
| Species Reactivity: | Human (Hu) Murine (Ms) Rat (Rt) Rabbit (Rb) Non-human primate (Nhp) Bovine (Bv) Ovine (Ov) Hamster (Hm) Porcine (Po) Ferret (Ft) |
| Immunogen: | Fusion protein containing residues 432-528 of human HIF-1alpha. |
| Ordering Information | ||||
| Pierce Anti-HIF-1 alpha Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| MA1-16504 | 250 µl | $340.00 | ||
| Storage: | 4º C, DO NOT FREEZE! |
| Form: | 250 µl of Protein G purified antibody (4 mg/ml) in PBS and 0.05% sodium azide. |
| Applications | Dilution |
| ChIP assay (ChIP) | Assay dependent |
| Immunocytochemistry (ICC) | Assay dependent |
| Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:25 - 1:100 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 - 1:100 |
| Immunoprecipitation (IP) | Assay Dependent |
| Western Blot (WB) | 1:500 |
| Product Specific Information |
| MA1-16504 detects HIF-1 alpha in human, monkey, sheep, mouse, rat, hamster, rabbit, porcine, bovine and ferret samples. MA1-16504 has successfully been used in Western blot, immunohistochemistry, immunocytochemistry, immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation applications. The HIF-1 alpha molecular weight is ~120 kDa. The MA1-16504 immunogen is residues 432-528 of human HIF-1alpha fusion protein. Figure 1 shows specific staining of human glioblastoma multiforme utilizing MA1-16504. Figure 2 shows Western blot analysis of 50 ug cobalt chloride induced COS-7 nuclear extracts using MA1-16504. |
| General Information |
| Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets. |
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Western Blot with anti-HIF-1 alpha Monoclonal Antibody [H1alpha67] (MA1-16504)
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Western Blot with anti-HIF-1 alpha Monoclonal Antibody [H1alpha67] (MA1-16504)
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(This product is for In Vitro experimental use only.)
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Box 117, Rockford, IL 61105 USA |
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