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CADHERIN E ANTIBODIES > CADHERIN E MONOCLONAL ANTIBODY MA1-06302
Anti-Cadherin E Monoclonal Antibody
Printable Datasheet
Synonyms:
Cadherin-1, Epithelial-cadherin, Uvomorulin, CAM 120/80, CD324 antigen
Entrez Gene ID:
(Human) 999
UniProt ID:
P12830
| Details | |
| Host / Isotype: | Mouse / IgG1 |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | 6F9 |
| Species Reactivity: | Human (Hu) |
| Immunogen: | E-cadherin derived from human vulva carcinoma cells. |
| Ordering Information | ||||
| Pierce Anti-Cadherin E Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| MA1-06302 | 100 µg | $343.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µg of antibody (1 mg/ml) in PBS with 0.09% sodium azide. |
| Applications | Dilution |
| Immunocytochemistry (ICC) | Assay dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:25-1:100 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25-1:100 |
| Western Blot (WB) | 1:50-1:500 |
| Product Specific Information |
| MA1-06302 detects E-cadherin in human samples. MA1-06302 has sucessfully been used in Western blotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry (frozen sections). By Western blotting, it detects a ~120 kDa protein representing E-cadherin. The MA1-06302 immunogen is affinity purified ~80 kD extracellular fragments of E-cadherin derived from tryptic digestion of A-431 human vulva carcinoma cells. |
| General Information |
| Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a ~120 kD transmembrane glycoprotein consisting of an ~80 kD extracellular and a ~40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighbouring cells. In addition, E-cadherin can interact heterophilically with integrin alphaE-beta7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia. |
(This product is for In Vitro experimental use only.)
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